mab mouse anti rig i Search Results


anti rig i  (OriGene)
90
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goat anti mda5  (Santa Cruz Biotechnology)
95
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anti rig i monoclonal antibody alme 1  (Cell Signaling Technology Inc)
96
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Anti Rig I Monoclonal Antibody Alme 1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rabbit anti rig i  (ProSci Incorporated)
91
ProSci Incorporated rabbit anti rig i
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immunodetection rabbit anti lgp2  (Proteintech)
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Proteintech immunodetection rabbit anti lgp2
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mouse mab against rig-i alme-1  (Enzo Biochem)
90
Enzo Biochem mouse mab against rig-i alme-1
Mouse Mab Against Rig I Alme 1, supplied by Enzo Biochem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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gene exp cxcl9 hs00171065 m1  (Thermo Fisher)
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Thermo Fisher gene exp cxcl9 hs00171065 m1
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gene exp cxcl10 mm00445235 m1  (Thermo Fisher)
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Thermo Fisher gene exp cxcl10 mm00445235 m1
Cytokine accumulation in the lungs of rMERS-MA-Δ5-infected hDPP4-KI mice. (A) Selected cytokine mRNA accumulation. Total RNA was extracted from lung samples, collected at 4 and 6 dpi, of mock-infected animals (gray) or animals infected with parental rMERS-MA (black) or mutant rMERS-MA-Δ5 (white) virus. Quantification of mRNAs encoding IFN-β, ISG15, IL-10, CCL4, CCL2, and <t>CXCL10</t> was performed by RT-qPCR using specific TaqMan assays; relative mRNA levels were based on comparison with mock-infected animals. (B) The levels of IFN-β in extracts from lung from mock-infected animals (gray) or animals infected with parental rMERS-MA (black) or mutant rMERS-MA-Δ5 (white) viruses were quantified by ELISA. Means from three animals per point are shown; error bars represent SEMs. r.u., relative units. *, P < 0.05; **, P < 0.01.
Gene Exp Cxcl10 Mm00445235 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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goat anti rig i  (Santa Cruz Biotechnology)
99
Santa Cruz Biotechnology goat anti rig i
Cytokine accumulation in the lungs of rMERS-MA-Δ5-infected hDPP4-KI mice. (A) Selected cytokine mRNA accumulation. Total RNA was extracted from lung samples, collected at 4 and 6 dpi, of mock-infected animals (gray) or animals infected with parental rMERS-MA (black) or mutant rMERS-MA-Δ5 (white) virus. Quantification of mRNAs encoding IFN-β, ISG15, IL-10, CCL4, CCL2, and <t>CXCL10</t> was performed by RT-qPCR using specific TaqMan assays; relative mRNA levels were based on comparison with mock-infected animals. (B) The levels of IFN-β in extracts from lung from mock-infected animals (gray) or animals infected with parental rMERS-MA (black) or mutant rMERS-MA-Δ5 (white) viruses were quantified by ELISA. Means from three animals per point are shown; error bars represent SEMs. r.u., relative units. *, P < 0.05; **, P < 0.01.
Goat Anti Rig I, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1 ap  (Proteintech)
95
Proteintech 1 ap
Cytokine accumulation in the lungs of rMERS-MA-Δ5-infected hDPP4-KI mice. (A) Selected cytokine mRNA accumulation. Total RNA was extracted from lung samples, collected at 4 and 6 dpi, of mock-infected animals (gray) or animals infected with parental rMERS-MA (black) or mutant rMERS-MA-Δ5 (white) virus. Quantification of mRNAs encoding IFN-β, ISG15, IL-10, CCL4, CCL2, and <t>CXCL10</t> was performed by RT-qPCR using specific TaqMan assays; relative mRNA levels were based on comparison with mock-infected animals. (B) The levels of IFN-β in extracts from lung from mock-infected animals (gray) or animals infected with parental rMERS-MA (black) or mutant rMERS-MA-Δ5 (white) viruses were quantified by ELISA. Means from three animals per point are shown; error bars represent SEMs. r.u., relative units. *, P < 0.05; **, P < 0.01.
1 Ap, supplied by Proteintech, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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resource source identifier antibodies anti rig i cell signaling technology  (Cell Signaling Technology Inc)
97
Cell Signaling Technology Inc resource source identifier antibodies anti rig i cell signaling technology
Cytokine accumulation in the lungs of rMERS-MA-Δ5-infected hDPP4-KI mice. (A) Selected cytokine mRNA accumulation. Total RNA was extracted from lung samples, collected at 4 and 6 dpi, of mock-infected animals (gray) or animals infected with parental rMERS-MA (black) or mutant rMERS-MA-Δ5 (white) virus. Quantification of mRNAs encoding IFN-β, ISG15, IL-10, CCL4, CCL2, and <t>CXCL10</t> was performed by RT-qPCR using specific TaqMan assays; relative mRNA levels were based on comparison with mock-infected animals. (B) The levels of IFN-β in extracts from lung from mock-infected animals (gray) or animals infected with parental rMERS-MA (black) or mutant rMERS-MA-Δ5 (white) viruses were quantified by ELISA. Means from three animals per point are shown; error bars represent SEMs. r.u., relative units. *, P < 0.05; **, P < 0.01.
Resource Source Identifier Antibodies Anti Rig I Cell Signaling Technology, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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nf κb pathway sampler kit  (Cell Signaling Technology Inc)
94
Cell Signaling Technology Inc nf κb pathway sampler kit
Cytokine accumulation in the lungs of rMERS-MA-Δ5-infected hDPP4-KI mice. (A) Selected cytokine mRNA accumulation. Total RNA was extracted from lung samples, collected at 4 and 6 dpi, of mock-infected animals (gray) or animals infected with parental rMERS-MA (black) or mutant rMERS-MA-Δ5 (white) virus. Quantification of mRNAs encoding IFN-β, ISG15, IL-10, CCL4, CCL2, and <t>CXCL10</t> was performed by RT-qPCR using specific TaqMan assays; relative mRNA levels were based on comparison with mock-infected animals. (B) The levels of IFN-β in extracts from lung from mock-infected animals (gray) or animals infected with parental rMERS-MA (black) or mutant rMERS-MA-Δ5 (white) viruses were quantified by ELISA. Means from three animals per point are shown; error bars represent SEMs. r.u., relative units. *, P < 0.05; **, P < 0.01.
Nf κb Pathway Sampler Kit, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Cytokine accumulation in the lungs of rMERS-MA-Δ5-infected hDPP4-KI mice. (A) Selected cytokine mRNA accumulation. Total RNA was extracted from lung samples, collected at 4 and 6 dpi, of mock-infected animals (gray) or animals infected with parental rMERS-MA (black) or mutant rMERS-MA-Δ5 (white) virus. Quantification of mRNAs encoding IFN-β, ISG15, IL-10, CCL4, CCL2, and CXCL10 was performed by RT-qPCR using specific TaqMan assays; relative mRNA levels were based on comparison with mock-infected animals. (B) The levels of IFN-β in extracts from lung from mock-infected animals (gray) or animals infected with parental rMERS-MA (black) or mutant rMERS-MA-Δ5 (white) viruses were quantified by ELISA. Means from three animals per point are shown; error bars represent SEMs. r.u., relative units. *, P < 0.05; **, P < 0.01.

Journal: Journal of Virology

Article Title: Middle East Respiratory Syndrome Coronavirus Gene 5 Modulates Pathogenesis in Mice

doi: 10.1128/JVI.01172-20

Figure Lengend Snippet: Cytokine accumulation in the lungs of rMERS-MA-Δ5-infected hDPP4-KI mice. (A) Selected cytokine mRNA accumulation. Total RNA was extracted from lung samples, collected at 4 and 6 dpi, of mock-infected animals (gray) or animals infected with parental rMERS-MA (black) or mutant rMERS-MA-Δ5 (white) virus. Quantification of mRNAs encoding IFN-β, ISG15, IL-10, CCL4, CCL2, and CXCL10 was performed by RT-qPCR using specific TaqMan assays; relative mRNA levels were based on comparison with mock-infected animals. (B) The levels of IFN-β in extracts from lung from mock-infected animals (gray) or animals infected with parental rMERS-MA (black) or mutant rMERS-MA-Δ5 (white) viruses were quantified by ELISA. Means from three animals per point are shown; error bars represent SEMs. r.u., relative units. *, P < 0.05; **, P < 0.01.

Article Snippet: All experiments and data analysis were MIQE compliant ( 68 ). table ft1 table-wrap mode="anchored" t5 TABLE 3 caption a7 Species Gene symbol b Description Assay ID c Mouse CCL2 Monocyte chemotactic protein 1 (MCP-1) Mm00441242_m1 CCL4 Macrophage inflammatory protein 1β (MIP-1β) Mm00443111_m1 CCL5 C-C motif chemokine 5, RANTES Mm01302428_m1 CXCL10 Interferon gamma-induced protein 10 (IP-10) Mm00445235-m1 DDX58 DEAD box protein 58, RIG-I Mm01216853_m1 IFNB1 Interferon beta (IFN-β) Mm00439552_s1 IFNG Interferon gamma (IFN-γ) Mm01168134-m1 IL1A Interleukin-1 alpha (IL-1α) Mm00439620-m1 IL1B Interleukin-1 beta (IL-1β) Mm01336189_m1 IL6 Interleukin-6 Mm00446190_m1 IL10 Interleukin-10 Mm00439614_m1 IRF1 Interferon regulatory factor 1 Mm01288580_m1 ISG15 Ubiquitin-like protein ISG15 Mm01705338_s1 MX1 Interferon-induced GTP-binding protein Mx1 Mm00487796_m1 TNF Tumor necrosis factor (TNF-α) Mm00443258_m1 Human CCL2 Monocyte chemotactic protein 1 (MCP-1) Hs00234140_m1 CCL4 Macrophage inflammatory protein 1β (MIP-1β) Hs99999148_m1 CXCL10 Interferon gamma induced protein 10 (IP-10) Hs00171042_m1 HMBS Hydroxymethylbilane synthase Hs00609297_m1 IFNB1 Interferon beta (IFN-β) Hs02621180_s1 ISG15 Ubiquitin-like protein ISG15 Hs01921425_s1 TNF Tumor necrosis factor alpha (TNF-α) Hs99999043_m1 Open in a separate window a Species-specific assays were used to quantify cellular mRNAs. b In agreement with the Human Genome Organization (HUGO) Gene Nomenclature Committee (HGNC; https://www.genenames.org ). c Gene expression assays were from ThermoFisher Scientific.

Techniques: Infection, Mutagenesis, Virus Quantification, Quantitative RT-PCR, Enzyme-linked Immunosorbent Assay

Cytokine mRNA accumulation in rMERS-CoV-Δ5-infected cells. MRC-5 cells were mock infected (gray) or infected at an MOI of 1 with mutant rMERS-CoV-Δ5 (white) or parental rMERS-CoV-WT (black) virus. Total RNA was extracted at the indicated times postinfection. Quantification of mRNAs encoding IFN-β, ISG15, CXCL10, CCL2, CCL4, and TNF was performed by RT-qPCR using specific TaqMan assays; relative mRNA levels were based on comparison with mock-infected cells. Means from three independent infections per point are shown; error bars represent SEMs. r.u., relative units. *, P < 0.05; **, P < 0.01; ***, P < 0.001.

Journal: Journal of Virology

Article Title: Middle East Respiratory Syndrome Coronavirus Gene 5 Modulates Pathogenesis in Mice

doi: 10.1128/JVI.01172-20

Figure Lengend Snippet: Cytokine mRNA accumulation in rMERS-CoV-Δ5-infected cells. MRC-5 cells were mock infected (gray) or infected at an MOI of 1 with mutant rMERS-CoV-Δ5 (white) or parental rMERS-CoV-WT (black) virus. Total RNA was extracted at the indicated times postinfection. Quantification of mRNAs encoding IFN-β, ISG15, CXCL10, CCL2, CCL4, and TNF was performed by RT-qPCR using specific TaqMan assays; relative mRNA levels were based on comparison with mock-infected cells. Means from three independent infections per point are shown; error bars represent SEMs. r.u., relative units. *, P < 0.05; **, P < 0.01; ***, P < 0.001.

Article Snippet: All experiments and data analysis were MIQE compliant ( 68 ). table ft1 table-wrap mode="anchored" t5 TABLE 3 caption a7 Species Gene symbol b Description Assay ID c Mouse CCL2 Monocyte chemotactic protein 1 (MCP-1) Mm00441242_m1 CCL4 Macrophage inflammatory protein 1β (MIP-1β) Mm00443111_m1 CCL5 C-C motif chemokine 5, RANTES Mm01302428_m1 CXCL10 Interferon gamma-induced protein 10 (IP-10) Mm00445235-m1 DDX58 DEAD box protein 58, RIG-I Mm01216853_m1 IFNB1 Interferon beta (IFN-β) Mm00439552_s1 IFNG Interferon gamma (IFN-γ) Mm01168134-m1 IL1A Interleukin-1 alpha (IL-1α) Mm00439620-m1 IL1B Interleukin-1 beta (IL-1β) Mm01336189_m1 IL6 Interleukin-6 Mm00446190_m1 IL10 Interleukin-10 Mm00439614_m1 IRF1 Interferon regulatory factor 1 Mm01288580_m1 ISG15 Ubiquitin-like protein ISG15 Mm01705338_s1 MX1 Interferon-induced GTP-binding protein Mx1 Mm00487796_m1 TNF Tumor necrosis factor (TNF-α) Mm00443258_m1 Human CCL2 Monocyte chemotactic protein 1 (MCP-1) Hs00234140_m1 CCL4 Macrophage inflammatory protein 1β (MIP-1β) Hs99999148_m1 CXCL10 Interferon gamma induced protein 10 (IP-10) Hs00171042_m1 HMBS Hydroxymethylbilane synthase Hs00609297_m1 IFNB1 Interferon beta (IFN-β) Hs02621180_s1 ISG15 Ubiquitin-like protein ISG15 Hs01921425_s1 TNF Tumor necrosis factor alpha (TNF-α) Hs99999043_m1 Open in a separate window a Species-specific assays were used to quantify cellular mRNAs. b In agreement with the Human Genome Organization (HUGO) Gene Nomenclature Committee (HGNC; https://www.genenames.org ). c Gene expression assays were from ThermoFisher Scientific.

Techniques: Infection, Mutagenesis, Quantitative RT-PCR

TaqMan assay results a

Journal: Journal of Virology

Article Title: Middle East Respiratory Syndrome Coronavirus Gene 5 Modulates Pathogenesis in Mice

doi: 10.1128/JVI.01172-20

Figure Lengend Snippet: TaqMan assay results a

Article Snippet: All experiments and data analysis were MIQE compliant ( 68 ). table ft1 table-wrap mode="anchored" t5 TABLE 3 caption a7 Species Gene symbol b Description Assay ID c Mouse CCL2 Monocyte chemotactic protein 1 (MCP-1) Mm00441242_m1 CCL4 Macrophage inflammatory protein 1β (MIP-1β) Mm00443111_m1 CCL5 C-C motif chemokine 5, RANTES Mm01302428_m1 CXCL10 Interferon gamma-induced protein 10 (IP-10) Mm00445235-m1 DDX58 DEAD box protein 58, RIG-I Mm01216853_m1 IFNB1 Interferon beta (IFN-β) Mm00439552_s1 IFNG Interferon gamma (IFN-γ) Mm01168134-m1 IL1A Interleukin-1 alpha (IL-1α) Mm00439620-m1 IL1B Interleukin-1 beta (IL-1β) Mm01336189_m1 IL6 Interleukin-6 Mm00446190_m1 IL10 Interleukin-10 Mm00439614_m1 IRF1 Interferon regulatory factor 1 Mm01288580_m1 ISG15 Ubiquitin-like protein ISG15 Mm01705338_s1 MX1 Interferon-induced GTP-binding protein Mx1 Mm00487796_m1 TNF Tumor necrosis factor (TNF-α) Mm00443258_m1 Human CCL2 Monocyte chemotactic protein 1 (MCP-1) Hs00234140_m1 CCL4 Macrophage inflammatory protein 1β (MIP-1β) Hs99999148_m1 CXCL10 Interferon gamma induced protein 10 (IP-10) Hs00171042_m1 HMBS Hydroxymethylbilane synthase Hs00609297_m1 IFNB1 Interferon beta (IFN-β) Hs02621180_s1 ISG15 Ubiquitin-like protein ISG15 Hs01921425_s1 TNF Tumor necrosis factor alpha (TNF-α) Hs99999043_m1 Open in a separate window a Species-specific assays were used to quantify cellular mRNAs. b In agreement with the Human Genome Organization (HUGO) Gene Nomenclature Committee (HGNC; https://www.genenames.org ). c Gene expression assays were from ThermoFisher Scientific.

Techniques: TaqMan Assay